Table 1.

Primer Sequences Used for Real-Time RT-PCR Determinations

Gene	Accession		Primer sequence	Region	Product length
IL-1β	NM_031512	Fwd	5′-AATGCCTCGTGCTGTCTGACC-3′	471-588	118
		Rev	5′-TTGTCGTTGCTTGTCTCTCCTTG-3′
TNF-α	NM_012675	Fwd	5′-TCTTCTGTCTACTGAACTTCGGGG-3′	284-365	82
		Rev	5′-ATGGAACTGATGAGAGGGAGCC-3′
IL-6	NM_012589	Fwd	5′-CAAGAGACTTCCAGCCAGTTGC-3′	81-191	111
		Rev	5′-TGTTGTGGGTGGTATCCTCTGTG-3′
18S	X01117	Fwd	5′-CCAGTAAGTGCGGGTCATAAGC-3′	1650-1732	83
		Rev	5′CCATCCAATCGGTAGTAGCGAC-3′

IL-1β, interleukin-1 β; TNF-α, tumor necrosis factor α; IL-6, interleukin-6; 18S, rat ribosomal RNA subunit.