. Author manuscript; available in PMC: 2009 Jan 31.

Published in final edited form as: J Immunol Methods. 2007 Dec 4;330(1-2):64–74. doi: 10.1016/j.jim.2007.10.020

Table 1.

Instrument configuration and reagent panels for the study of lymphocyte subsets^a

Detector	Laser (nm)	Filter (nm)	Fluorochrome	CD8 T cell panel	NKT cell panel	NK cell panel
FL1	488	530/40	FITC	CD28	CD57	CD107a
FL2	488	575/25	PE	CD7	CD1d DimerX	NKG2C
FL3	488	613/20	PE-TR/QD605	CD62L	CD127	NKG2A
FL4	488	680/30	PerCp/PE-Cy5	CD8	CD161	CD56
FL5	488	750LP	PE-Cy7	CCR7	CD56	IFNγ
FL6	405	450/50	PacB	CD45RA	CD16	CD16
FL7	405	550/30	CasY	CD3	CD3	CD3
FL8	635	665/20	APC/Alexa 647	HLA-A2 tetramer	Vα24	TNFα
FL9	635	750LP	APC-Cy7	CD27	CD4	CD14

Reagents were from BD Biosciences (San Diego, CA, USA),Coulter Immunotech (Marseilles, France), Dako (Glostrup, Denmark) eBioscience (San Diego, CA, USA), Invitrogen (Eugene, OR, USA),One Lambda (Canoga Park, CA),Pierce (Rockford, IL, USA), R&D Systems (Minneapolis, MN, USA).