Table 2.
Compensation matrix for a flow cytometry panel with nine fluorescence parametersa
| Fluorochrome | Detector | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| FL1 | FL2 | FL3 | FL4 | FL5 | FL6 | FL7 | FL8 | FL9 | |
| FITC | 29 | 7.9 | 4.3 | 0.2 | 0 | 1.6 | 0 | 0 | |
| PE | 0.8b | 30.6 | 18.2 | 2.7 | 0 | 1.4 | 0 | 0 | |
| PE-TR/QD605 | 0.2/0 | 13/3.5c | 82/1 | 17/0 | 0/0 | 0/0.4 | 1/0.1 | 0/0 | |
| PE-Cy5/PerCP | 0/0 | 0.6/0 | 0.2/0 | 17.5/13.5 | 0/0 | 0/0 | 71/23.2 | 10.1/2.0 | |
| PE-Cy7 | 0 | 1.4 | 0.4 | 0.6 | 0 | 0 | 0 | 7.7 | |
| PacB | 0 | 0 | 0 | 0 | 0 | 9.0 | 0 | 0 | |
| CasY | 0 | 0 | 0 | 0 | 0 | 2.8 | 0 | 0 | |
| Alexa 647/APC | 0/0 | 0/0 | 0/0 | 0.4/0.6 | 0/0 | 0/0 | 0/0 | 13.4/9.3 | |
| APC-Cy7 | 0 | 0 | 0 | 0.3 | 2.1 | 0 | 0 | 10.0 | |
a
Compensation matrix generated in the FlowJo software.
b
A compensation particle set, consisting of uncoated polystyrene beads, or beads coated with anti-mouse Igκ (BD Biosciences), were stained with mAbs conjugated with each of the fluorochromes used.
c
Grey fields highlight where exchanging fluorochromes has the most significant effect on compensation needs.