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Comparison of the expression of Notch 1, Notch 4, and APP in in vitro CEC and human brain frontal cortex. Notch 1, Notch 4, and APP expression and localization were assessed by immunocytochemical labeling of cerebral endothelial cells (CEC) (top panels) and paraffin embedded human frontal cortex tissue (lower panels). CD31 was used as an endothelial cell-specific marker. Baseline immunoreactivity was observed by fluorescent confocal microscopy. Original magnification is 62×.
