Table 1.

Experimentally determined IC₅₀ values for the binding of monosubstituted CII261-273 analogs to HLA-DRB1^*0401

Peptide	Amino acid sequence and putative position in the binding pocket													IC50 (nM)
	P-1	P-2	P1	P2	P3	P4	P5	P6	P7	P8	P9	P10	P11
CII261-273	A	G	F	K	G	E	Q	G	P	K	G	E	P	180
CII262A	A	A	F	K	G	E	Q	G	P	K	G	E	P	52
CII263A	A	G	A	K	G	E	Q	G	P	K	G	E	P	>35,000
CII264A	A	G	F	A	G	E	Q	G	P	K	G	E	P	2022
CII265A	A	G	F	K	A	E	Q	G	P	K	G	E	P	21
CII266A	A	G	F	K	G	A	Q	G	P	K	G	E	P	1249
CII267A	A	G	F	K	G	E	A	G	P	K	G	E	P	115
CII268A	A	G	F	K	G	E	Q	A	P	K	G	E	P	65
CII269A	A	G	F	K	G	E	Q	G	A	K	G	E	P	781
CII270A	A	G	F	K	G	E	Q	G	P	A	G	E	P	77
CII271A	A	G	F	K	G	E	Q	G	P	K	A	E	P	40
CII272A	A	G	F	K	G	E	Q	G	P	K	G	A	P	24
CII273A	A	G	F	K	G	E	Q	G	P	K	G	E	A	429
CII263N	A	G	N	K	G	E	Q	G	P	K	G	E	P	>35,000
CII263S	A	G	S	K	G	E	Q	G	P	K	G	E	P	>35,000
CII263Y	A	G	Y	K	G	E	Q	G	P	K	G	E	P	541
CII266D	A	G	F	K	G	D	Q	G	P	K	G	E	P	2222
CII266K	A	G	F	K	G	K	Q	G	P	K	G	E	P	>35,000
CII266R	A	G	F	K	G	R	Q	G	P	K	G	E	P	>35,000