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. 2008 Feb 29;7:42. doi: 10.1186/1475-2875-7-42

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Copyright © 2008 Lucchi et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Migration of PBMC towards iRBC^ST-stimulated ST cells. Primary ST was stimulated with iRBC^ST, uRBC or left unstimulated for 12 hours. PBMCs added to the upper chamber of a dual chamber were allowed to migrate for 12 hours. Calcein AM-stained cells in 8 random fields for each stimulant were counted. The mean ± SD of migrated cells upon stimulation was compared to the spontaneous migration towards unstimulated ST (MED). Interaction with iRBC^STled to a significant migration of cells towards iRBC^ST-stimulated ST compare to both controls (* = P < 0.0040). Representative data from one of two experiments is shown.