Figure 1.
Creation of a transgenic mouse with forced expression of p57Kip2 in a cre dependent manner. (A) Recombination scheme displaying the structure of the targeting vector, genomic R26 locus, targeted locus after homologous recombination and recombined locus after cre activation (B) PCR genotype analysis of tail genomic DNA (gDNA) (C) PCR analysis shows that cre-induced recombination is restricted to the heart, and occurs only in the presence of cre recombinase. T= tail gDNA, H = heart gDNA (D) RT-PCR analysis demonstrates robust expression of p57Kip2 message in adult transgenic hearts in the presence of cre recombinase, compared to barely detectable levels in the absence of cre recombinase.