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. 2008 Apr 2;3(4):e1893. doi: 10.1371/journal.pone.0001893

Figure 3. Males and females call differently during male-female interaction in Exp. 2, 4 and 5.

Figure 3

(A) Representative sonograms of mouse ultrasonic vocalizations produced during male-female interaction. 4A1: flat USV, 4A2: frequency-modulated sine-wave-like USV and 4A3: broken USV, and 4A4: breached-harmonic USV. 4A5: human audible dense-layered squeaks. (B) No USVs were detected when male were silenced by simple mouth taping; but when females were silenced, many USVs were detected, suggesting that USVs recorded during male-female interaction were emitted from the male. (C) After aggressive attacks from male mice, females produced a few squeaks, observed on sonograms as harmonic dense-layered calls. (D) When reunited with another female after 4 hour's separation as described before [73], female mice emitted frequency-modulated USVs as those produced by male mice during male-female interaction. Three representative broken USVs were shown.