Fig. 2.
Spatio-temporal patterns of CCh-induced [Ca2+]i transients. The x–y confocal Ca2+ imaging was performed at 32 Hz (A and B), 40 Hz (C), 44 Hz (D) and 30 Hz (E). For each cell, the time course plot of the normalized fluo-4 fluorescence intensity was averaged (red trace) within sub-PM regions of interest where Ca2+ waves (induced by 10 μM CCh) were initiated (insets), and (green trace) within the total confocal optical slice of the SMC. The galleries below the plots show sequential confocal images (after rotation by 90°) captured during the periods highlighted by grey background in the plots. Magenta arrowheads in the galleries indicate sub-PM [Ca2+]i upstrokes.