Fig. 7.
Summary of the effects of VGCC/SERCA/RyR/IP3R inhibitors on the initial phase of CCh-induced [Ca2+]i transient. Experimental protocol is illustrated in (A). The fluo-4 loaded SMCs were stimulated with 600-ms pulses of 10 μM CCh applied with a10 min interval. The response (ΔF/F0 averaged at multiple sub-PM regions) to the second CCh application (Test) was related to the response to the first CCh application (Control). The Test response was obtained either in the control (a), or following incubation with a drug (b–d) or a combination of several drugs. Two parameters were examined and summarized: (B) relative change in peak amplitude (ΔF/F0)Test/(ΔF/F0)Control, and (C) relative change in time-to-peak, (tpeak)Test/(tpeak)Control. The symbol (*) shows the significant difference (p < 0.0000002) between the parameters in control external solution and in the presence of drug (or drug combination), as indicated.