Figure 9. The LG domains of laminin-332 increase the osteogenic-specific marker of calcified matrix in hMSC.
Cells were cultured for 21 days on indicated substrates and stained for calcium using Alizarin Red (A). Colorimetric assessment of total calcium in hMSC (B). Cells were plated for 21 days as for panel A, then lysed and total calcium detected colorimetrically. Measurements were read at 570 nm.