Fig. 1.

p17R expression on human pDCs. (A) Flow cytometry was used to detect the expression of p17R on BDCA-2⁺ pDCs within freshly isolated PBMCs. (Upper) Results are displayed as bivariate dot plots. (Lower) pDCs were then purified, and p17R expression on their surface was evaluated on BDCA-2-gated cells (thick line). The thin line shows isotype control mAb. Immunocytochemical staining of p17R on purified pDCs is shown. (B) The expression of p17R was evident in freshly isolated cells. (C) The lymphoblastic cell lines H9 and Raji served as negative (Left) and positive (Right) control, respectively. (D) Lack of staining was evident when pDCs were cultured for 18–24 h. (B Left and D Left) Unbiotinylated protein was used as specificity control. Representative results from one of three independent experiments are shown.