Table 2.
Q-TOF analysis of tryptic glycopeptides from the purified, assembled 2G12 antibody isolated from maize seed endosperm and the 40-kDa degradation product thereof
| Structure | Intensity |
|
|---|---|---|
| Full-size 2G12 | Degraded 2G12 | |
| Nonglycosylated | 11.5 | 16.1 |
| Oligomannose-type N-glycans | 5.9 | nd |
| Complex-type N-glycans | 27.9 | 67.0 |
| Single GlcNAc | 54.6 | 16.9 |
The group of oligomannose-type glycans consisted of the N-glycans Man5GlcNAc2 to Man9GlcNAc2 in similar orders of magnitude. Complex-type N-glycans comprised the structures MUXF3, MMXF3, GnMXF3/MGnXF3, and GnGnXF3 in roughly similar amounts in both samples. nd, none detected.