Fig. 1.

A mouse model in which T cells are MyD88-deficient, but MyD88-dependent innate responses are intact. (A) Scheme representing the hematopoietic reconstitution strategy. (B) Twelve weeks after reconstitution, T cells were isolated from mice (>90% purity), and an immunoblot was performed to assess MyD88 expression. (C) Chimeric mice were generated as illustrated in A, except that the Myd88^−/− mice were CD45.1+, whereas the other marrow donors and the recipients were CD45.2+. After 12 weeks, flow cytometry was performed on PBMCs; data represent two individually reconstituted cohorts. (D) Splenocytes were cultured for 24 h in the presence or absence of CpG DNA and then analyzed by flow cytometry for B cell expression of CD86. Open histogram, CpG DNA stimulated; shaded histogram, unstimulated. These data are representative of three experiments. (E) Mice from each cohort were injected with STAg, and serum was obtained after 3 h for ELISA; these data are representative of three experiments; N.D., none detected. For all experiments, unless otherwise indicated, data represent at least three experiments.