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Degeneration of hypothalamic neurons in adult Ubb−/− mice. In situ hybridization of AgRP (A) and NSE (B) mRNA from Ubb−/− mice (n = 3 or 4) and littermate controls (n = 3 or 4). Images are representative for each age and group. For quantification, stereological counts were obtained from eight serial sections generated from each brain. 3V, third ventricle. The data are expressed as means ± SEM from the indicated number of mice. ∗, P < 0.05.
