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. 2008 Mar 3;105(10):3837–3842. doi: 10.1073/pnas.0712250105

Fig. 3.

Fig. 3.

Identification of the LB-PI4K2B-1S epitope recognized by CD4+ T cell clone ZRZ38. (A) A series of peptides comprising the amino acids 64–92 region of LB-PI4K2B-1S were synthesized, pulsed onto donor EBV-LCLs, and tested for recognition by CD4+ T cell clone ZRZ38 in IFN-γ ELISA. Indicated is the S/P residue at position 78 (shaded) and the minimal 12-mer SRSSSAELDRSR peptide (amino acids 74–85, boxed) recognized by clone ZRZ38. The mean release of IFN-γ (ng/ml) in 50-μl supernatants of duplicate wells is shown. (B) PBMCs from HLA-DQB1*0603+ healthy individuals were genotyped for the LB-PI4K2B-1S/P polymorphism and tested for T cell recognition in IFN-γ ELISA (filled bars). As a control for cell viability and HLA-DQB1*0603 expression, PBMCs were tested for T cell recognition after 2 h of pulsing with the 12-mer LB-PI4K2B-1S peptide (open bars). Genotype data are shown as +/+ (S/S), +/− (S/P), and −/− (P/P). The mean release of IFN-γ (pg/ml) in 50-μl supernatants of duplicate wells is shown.