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. 2008 Feb 27;105(10):3785–3789. doi: 10.1073/pnas.0800546105

Fig. 3.

Fig. 3.

TDP-43 depletion promotes pRb and p130 phosphorylation and causes changes in cell cycle distribution. (A) Western blot analysis to detect pRb and pRb2/p130 levels in U2OS cell extracts from TDP-43 depleted (lanes 2 and 4) and control treated cells (lanes 1 and 3). Extracts were also treated with λ phosphatase (PPTase) (lanes 3 and 4) to compare unphosphorylated pRb and pRb2/p130 protein levels. Tubulin was used as loading control. (B) Bars show the percentage of cells in G0/G1, S, and G2/M phases of the cell cycle in proliferating siRNATDP-43 and siRNA control-treated U2OS cells. Propidium- and BrdU-labeled cells were analyzed by FACS after RNAi treatment. Results show average values of three independent experiments, and error bars show SD.