Figure 6.

Effect of anti-MDR-1 mAb on the maturation of DC. The levels of MHC II expressed by epidermal DC were analyzed in gated epidermal suspensions by flow cytometry before the onset of culture (Day 0) or after 3 days of culture in the absence of mAb or in the presence of MRK16. Single cell suspensions of the epidermis were prepared by digestion with dispase followed by trypsin. Keratinocytes and other skin cells were excluded from the analysis by setting a gate to include only MHC II⁺ cells.