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. 2008 Mar 3;105(10):3963–3967. doi: 10.1073/pnas.0709530105

Fig. 3.

Fig. 3.

Cryo-electron micrographs of vitreous cryosections from mycobacteria. The sections have a nominal thickness of 35 nm. (A) Cross-section of an M. smegmatis cell deformed by the cutting process. Regions perpendicular to the cutting direction (arrowheads) were used for further analyses. (Scale bar: 200 nm.) (B) Cell envelope of M. smegmatis (subarea from A). (C) Cell envelope of M. bovis bacillus Calmette–Guérin. (Scale bars: 100 nm.) (D and E) Averaged profiles from the cell envelopes of M. smegmatis (D) and M. bovis bacillus Calmette–Guérin (E). CM, cytoplasmic membrane; L1 and L2, domain-rich periplasmic layers; MOM, mycobacterial outer membrane. Note that the distances between the membranes and layers are influenced by the cutting process. The bilayer structure of the CM and the MOM is discernible (B–E). Images are corrected for the contrast transfer function with fitted defocus values of −6.4 μm (B) and −6.7 μm (C).