Table 2.

Transdifferentiation of Y to PDA does not occur in egl-5 and sem-4 mutants

Relevant genotype	2 P11.p, % (n)	Persistent Y, % (n)	No PDA, % (n)
Wild type	0 (34)	0 (34)	5.8 (51)
egl-5(n945)	97.5 (41)	100 (41)	100 (34)
sem-4(n1971)	12.5 (32)	84.8 (79)*	100 (38)

(n), total number of L3 and older hermaphrodites scored; 2 P11.p, percentage of animals with 2 P11.p-like cells; persistent Y, percentage of animals in which Y remained at its initial position with an epithelial appearance and expressed an epithelial or a Y marker. ajm-1::gfp was used to score egl-5 mutants and egl-26::gfp was used for sem-4 mutants; No PDA, percentage of animals that did not express ace-3/4::gfp.

*In 15% sem-4 mutants, P12.pa is not formed or does not differentiate as a rectal epithelial cell, as assessed by using both egl-26::gfp and egl-5::gfp reporters. This result suggests that hermaphrodites scored here as lacking a persistent Y probably had one but no recognizable P12.pa consistent with the results obtained with a PDA marker.