Table 3.
The ectopic Y cell in lin-12 mutants, but not in egl-38 or mab-9 mutants, undergoes transdifferentiation
| Genotype | 2 PDA in L4, % (n) | B GFP + in L1, % (n) | B GFP + in L4, % (n) | U GFP + in L1, % (n) | U GFP + in L4, % (n) |
|---|---|---|---|---|---|
| egl-26::gfp (Y marker) | — | 100 (39) | 100 (34) | 25 (39) | 0 (34) |
| mab-9(e2410);egl-26::gfp | — | 93 (30)* | 88 (51)* | — | — |
| egl-38(sy294);egl-26::gfp | — | — | — | 100 (21)† | 74 (23)† |
| cog-1::gfp (PDA marker) | 0 (59) | — | 0 (59) | — | — |
| mab-9(e2410);cog-1::gfp | 0 (37) | — | 0 (37)* | — | — |
| ace-3/4::gfp (PDA marker) | 0 (131) | — | — | — | 0 (51) |
| egl-38(sy294); ace-3/4::gfp | 0 (95) | — | — | — | 0 (95)† |
| lin-12(n137); ace-3/4::gfp | 76 (54)‡ | — | — | — | — |
The percentage of newly hatched (L1) or L3 to adult (collectively called L4) hermaphrodites were scored for the presence of a GFP-positive cell at the position of the extra Y; (n), total number of animal scored.
*The cell at B position forming the extra Y cell was scored.
†The cell at U position forming the extra Y cell was scored.
†In some lin-12(n137) mutant hermaphrodites (20/54), the axon of one or both of the neurons expressing the PDA marker went more posteriorly than it does in wild type before joining the dorsal cord, perhaps reflecting an effect of elevated LIN-12/Notch activity on axon guidance.