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Measurement of oligomannose dendrons–2G12 complex interaction by glycan array competition assay. (a) Design of glycan array-based competition assay (not drawn to scale). (b) Representative microarray slide. 16 (bottom row) and 17 (top row) are used as inhibitors; concentrations from left to right are 50 μM, 5 μM, 500 nM, 50 nM, 5 nM, 500 pM, 50 pM, and 0. (Scale bar: 1 mm.) (c) Inhibition curves of 16 and 17 for the determination of solution dissociation constant.
