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CD8+ T cell clones specific for HLA-A1 CMV-pp65 (Left) and HLA-A3 EBV-EBNA-3a (Right) were stained with equal amounts of HLA-A1 CMV-pp65 or HLA-A3 EBV-EBNA-3a tetramers, generated via peptide exchange (Exchanged, Upper) or classical refolding (Traditional, Lower). Control cells represent staining with the matched MHC tetramer and cross-sample controls.
