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To determine the kinetics of Hb transport in Rab7 and its mutants overexpressed Leishmania, cells were allowed to bind Alexa Fluor 594-conjugated Hb at 4°C and subsequently chased for indicated times at 23°C as described in Materials and Methods. Finally, cells were fixed with paraformaldehyde and visualized under confocal microscope. Results are the representative of three independent observations.
