Figure 6.
Discrimination of the early apoptotic, late apoptotic, and necrotic cell populations using the Annexin/V-7 AAD test on Chang (WKD) and IOBA-NHC cells. Annexin/V-7 AAD staining results (flow cytometry) show the early apoptotic, late apoptotic, and necrotic cell populations after 15 min of treatment with different concentrations of BAC on (A) Chang (WKD) and (B) IOBA-NHC cells. Note that BAC toxicity (late apoptosis) was found with BAC at 10−5% and 10−4% in Chang cells whereas on IOBA-NHC cells, the toxicity was only found with BAC at 10−3% and higher, and that with BAC at 5.10−3%, most of IOBA-NHC cells were in a late apoptotic process whereas most of Chang cells were necrotic. Statistical results are not shown (developed in the results section). BAC, benzalkonium chloride; PBS, phosphate buffered saline. Means ± SEM.