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. Author manuscript; available in PMC: 2008 Dec 15.
Published in final edited form as: Toxicon. 2007 Jul 31;50(8):1027–1040. doi: 10.1016/j.toxicon.2007.07.010

FIGURE 2.

FIGURE 2

(A) Dose and time response of C. difficile toxin A (0.001-3μg/mL) exposure on IEC-6 cell proliferation following 24 hours, showing inhibition of cell proliferation in vitro. Cell proliferation assay was assessed by reading the absorbance using an ELISA microplate reader at 450nm in 96-well, following 24 hour of C. difficile toxin A exposure. After 24 hours, wells were incubated for 2 hours with 10μL of tetrazolium salt and the absorbance was measured. *P<0.05 compared with the control containing non-exposed IEC-6 cells seeded in a standard medium (C), by ANOVA and Bonferroni's test. (B) Effect of retinol (vit A) on cell proliferation assay by detected absorbance using an ELISA microplate reader at 450nm in 96-well seeded IEC-6 cells. Retinol (0.01-100nM) was diluted in medium without glutamine. After 24hours, wells were incubated for 2 hours with 10uL of the tetrazolium salt and the absorbance was measure at 24 hours. *P<0.05 compared to the non-treated and toxin A exposed control (TxA), by ANOVA and Bonferroni's test.