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. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: Free Radic Biol Med. 2007 Dec 5;44(5):893–906. doi: 10.1016/j.freeradbiomed.2007.11.015

Figure 1.

Figure 1

Detection of myoglobin and hemoglobin radical-derived nitrone adducts by immuno-spin trapping. Reaction mixtures contained globin (500 μM), DMPO (100 mM) and H2O2 (500 μM), or subsets of these as indicated. The reactions were initiated by adding H2O2 to the mixture of globin and the spin trap. After 10 min incubation at 25 °C, reactions were diluted with deionized H2O and the globin radical-derived nitrone adducts were detected by ELISA. Values are mean ± S.D (n = 3).