Skip to main content
. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: Free Radic Biol Med. 2007 Dec 5;44(5):893–906. doi: 10.1016/j.freeradbiomed.2007.11.015

Figure 2.

Figure 2

Effects of chaotropes, organic solvents and solvent modifiers on nitrone adduct stability. Reaction mixtures containing the Mb-derived nitrone adducts with and without the indicated concentration of chaotropes, organic solvents or organic modifiers were incubated for 14 hours at 37 ºC. Results obtained with HCl are for a 30 s incubation at 25 ºC. At the conclusion of the experiments, reactions were diluted with coating buffer for ELISA analysis as described under Materials and Methods. Gu.HCl, guanidine hydrochloride; FA, formic acid; TFA, trifluoroacetic acid; AA, acetic acid. Values are mean ± S.D (n = 3).