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. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: Free Radic Biol Med. 2007 Dec 5;44(5):893–906. doi: 10.1016/j.freeradbiomed.2007.11.015

Table 1.

Summary of identified peptides originating from proteolytic digestion of human hemoglobin, horse heart myoglobin and sperm whale myoglobin.

Peaka Elution Time (min) Proteinb Proteasec Amino acid sequence d Res.Mod.e Observed f (m/z) Calculated f (m/z)
1 42.34 HuHb Try Gly83-Lys95 (α) Cys93 766.86 766.87
2 42.65 HuHb Try Gly83-Lys95 (α) n.m. 711.34 711.34
3 44.50 HuHb Try Thr41-Lys56 (α) n.m. 917.43 917.45
4 44.84 HuHb Try Thr41-Lys56 (α) Tyr42 972.96/917.43 g 972.98/917.45 g
5 46.06 HuHb Try Thr41-Lys56 (α) His45 972.99 972.98
6 47.37 HuHb Try Thr41-Lys56 (α) His50 973.01 972.98
7 64.73 HoMb Try Tyr103-Lys118 Tyr103 998.55/943.09 g 998.55/943.02 g
8 65.11 HoMb Try Tyr103-His116 Tyr103 890.95/835.56 g 890.99/835.45 g
9 66.56 HoMb Try Tyr103-Lys118 n.m. 942.80 943.02
10 66.86 HoMb Try Tyr103-His116 n.m. 835.26 835.45
11 68.76 HoMb Try Tyr103-Lys118 His116 998.52 998.55
12 69.01 HoMb Try Tyr103-His116 His113 890.98 890.99
13 71.80 HoMb Try Tyr103-His116 His116 890.98 890.99
14 40.52 HoMb Chy Ala90-Leu104 Tyr103 923.51/867.98 g 923.54/868.00 g
15 42.96 HoMb Chy Lys98-Leu104 Tyr103 493.28/437.73 g 493.33/437.79 g
16 47.27 HoMb Chy Ala90-Phe106 Tyr103 1061.55/1006.03 g 1061.59/1006.06 g
17 47.61 HoMb Chy Lys96-Phe106 Tyr103 763.94/708.40 g 763.96/708.42 g
18 50.16 HoMb Chy Lys98-Phe106 Tyr103 631.34/575.80 g 631.38/575.85 g
19 40.65 SwMb Chy Ala90-Leu104 Tyr103 923.61/868.08 g 923.54/868.00 g
20 47.29 SwMb Chy Ala90-Phe106 Tyr103 1061.69/1006.16 g 1061.59/1006.06 g
21 47.63 SwMb Chy Lys96-Phe106 Tyr103 764.02/708.49 g 763.96/708.42 g
22 50.20 SwMb Chy Lys98-Phe106 Tyr103 631.42/575.87 g 631.38/575.85 g
n.p. 67.28 SwMb Try Tyr103-His116 Tyr103 897.98/842.47 g 897.99/842.46 g
n.p. 67.28 SwMb Try Tyr103-Arg118 Tyr103 1019.57/964.03 g 1019.56/964.03 g
n.p. 71.23 SwMb Try Tyr103-Arg118 His116 1019.55 1019.56
n.p. 71.23 SwMb Try Tyr103- His116 His113 898.00 897.99
a

Peak numbers are assigned in the order of retention time as shown in Fig. 4 and 5; n.p., peptide maps not presented

b

Protein: HuHb, Human hemoglobin; HoMb, Horse myoglobin; SwMb; Sperm whale myoglobin.

c

Protease: Try, Trypsin; Chy, Chymotrypsin.

d

(α), α chain; (β), β chain.

e

Res. Mod., residue modified by DMPO; n.m., not modified.

f

Observed and calculated m/z values: value are for +2 ions

g

Pairs of ions showing a mass-to-charge difference of approximately 55.5