Figure 3.

Double mutant hck^−/−fgr^−/− mice develop only mild tissue damage during endotoxic shock. Serum chemistry levels assessing liver damage (AST/ALT), renal function (BUN), and muscle/kidney damage (LDH) were determined in wild-type and double mutant mice at the indicated time points following i.p. injection of LPS (30 mg/ml). A total of 15 mice per group were injected, and blood was collected by retroorbital bleeding at the indicated times; however within 24 hr 9 wild-type mice had died and by 48 hr only 2 animals remained; therefore, these two time points reflect data averaged from fewer animals. Over the course of this experiment 13 hck^−/−fgr^−/− mutants survived completely (2 died before 48 hr; hence the final data points reflects results averaged from remaining animals). To facilitate comparison between wild-type and mutant animals, we determined the slopes of curves between the 0 and 24-hr time points for each analyte. For all analytes the slope was greater than 3-fold higher in wild-type (wt) than hck^−/−fgr^−/− animals (AST: wt = 31, mutant = 8; ALT: wt = 22, mutant = 7; BUN: wt = 2.9, mutant 0.6; LDH: wt = 203, mutant = 47). Error bars are SD.