Skip to main content
. Author manuscript; available in PMC: 2008 Mar 18.
Published in final edited form as: Hum Gene Ther. 2007 Mar;18(3):269–285. doi: 10.1089/hum.2006.186

Figure 1. Injection volumes between 2.5 and 10 ml are all suitable for EPO gene delivery into the skeletal muscle of adult rats.

Figure 1

A constant amount (500 μg) of mMCK-ratEPO pDNA was injected on day 0 into the hind limbs of adult Lewis rats in various volumes. The physiological effect of the expressed EPO was followed by analyzing the absolute changes in the animals’ hematocrit (Δ Hct) at various time points. Group averages and SEM are shown (n=10 animals per group). The asterisk marks the first time point (day 28) when significant differences between the 5 ml-injected group and the 10 or 2.5 ml-injected ones could be observed (p<0.05). While the 5 and 2.5 ml-injected groups remained significantly different across all time points (p=0.024), differences between the other EPO-injected groups did not reach statistical significance.