Table 1. Correction of anemia in a rat disease model by the repeated, small-dose injections of mMCK-ratEPO.

The anemia model was created as described for Figure 3. The Δ Hct values that were graphically illustrated on Fig. 3C are here presented in a table format for Groups 3–6, along with the p-values of the statistical analysis comparing these groups. The cumulative Empty-mMCK control and mMCK-RhEPO pDNA doses delivered by day 133, 168 and 203 are also shown. The last column reflects the maximum increase in hematocrit values in each group relative to the total amount of delivered plasmid DNA (Δ Hct/μg DNA).

The additive effect of repeated small-dose injections on hematocrit changes in a rat model of anemia
		Weeksa	0	1	4	8	Cumulative	9	10	13	Cumulative	14	15	16	18	Cumulative	Total gain: max
Group	Injected DNA	Daysb		84	105	133	DNA dose		147	168	DNA dose		182	189	203	DNA dose	Δ Hct/μg DNA
3	Empty-mMCK control	Δ Hct vs Day 63	1st injection on Day 77	0.8	3.5	2.7	50 μg Ctr.	2nd injection on Day 140	−1.5	0.7	75 μg Ctr.	3rd injection on Day 175	−2.2	−2.1	−3.5	100 μg Ctr.	0.035
4	25+25+25 μg mMCK-ratEPO	ΔHct vs Day 63		2.7	7.7	8.1	25 μg		4.7	9.9	50 μg		13.6	14.4	16.5	75 μg	0.220
5	50+25 μg mMCK-ratEPO	Δ Hct vs Day 63		4.0	10.2	12.0	50μg		11.2	14.2	75μg		13.0	15.1	15.7	100 μg	0.157
6	100 μg mMCK-ratEPO	Δ Hct vs Day 63		5.7	15.5	17.8	100 μg		15.9	15.3	100 μg		13.5	15.6	12.4	100 μg	0.178
Probability (p) values for the pairwise comparison of EPO-treated and control groups using Analysis of Variance (ANOVA)
3 vs 4					0.016	0.018				0.000					0.000
3 vs 5					0.000	0.000				0.000					0.000
3 vs 6					0.000	0.000				0.000					0.003
4 vs 5					0.142	0.107				0.108					0.730
4 vs 6					0.000	0.000				0.058					0.254
5 vs 6					0.004	0.015				0.727					0.458

^aWeeks are counted from the time of the 1 st DNA injections.

^bDays are counted from the time of drawing the first blood samples before initiating the study.