Fig. 5.
The efficient expansion of melan-A specific CD8+ T cells from healthy and HCV infected individuals. In three representative paired, controlled experiments, dsRNA matured MD-DCs from healthy (left hand panel; HI-5, -8, -9) and HCV infected (right hand panel; HCV-9, -10, -11) individuals were pulsed with 1 μg/mL melan-A26-35 peptide or RPMI medium, washed thoroughly and then incubated with autologous PBMC at a ratio of 1DC: 10 PBMCs. After 13 days, cultures were stained with melan-A tetramer-PE, CD 8-FITC and 7-AAD. DCs are gated on the live population. % tetramer+/CD8+ T cells is given in each FACS plot.