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. Author manuscript; available in PMC: 2008 Mar 19.
Published in final edited form as: Vet J. 2007 Feb 1;175(1):89–95. doi: 10.1016/j.tvjl.2006.12.001

Fig. 4.

Fig. 4

Effects of NSAID and PPARγ ligands on NAG-1 expression in canine osteosarcoma cancer cells determined by RT-PCR. (a) CCL-183 cells were treated for 24 h with DMSO vehicle (Veh) or NSAIDs: DFU: 5,5-dimethyl-3-(3-fluorophenyl)-4-(4-methylsulphonyl) phenyl-2 (5H)-furanonel, 100 μM; Dic: diclofenac, 100 μM; Pir: piroxicam, 100 μM; SC: SC-560, 50 μM; SS: sulindac sulphide, 30 μM. Plasmid containing canine NAG-1 cDNA was used as a positive control (Cont). (b) CCL-183 cells were treated for 24 h with vehicle (Veh) or PPARγ ligands: MCC: MCC-555, 5 μM; RGZ: rosiglitazone, 5 μM. RT-PCR was performed using total RNA isolated from cells. Equal loading was demonstrated by GAPDH RT-PCR.