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. 2008 Jan 30;67(6):1196–1210. doi: 10.1111/j.1365-2958.2007.06103.x

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© 2008 The Authors Journal compilation © 2008 Blackwell Publishing Ltd

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Fig. 6 — Complementation of the wt xer1 gene in PLMU. A. Schematic representation of complementation plasmid p21-7Gxer. Restriction sites used for cloning purposes are as indicated and arrowheads represent direction of transcription; ColE1, E. coli origin of replication; bla, ampicillin resistance gene; aacA-aphD, Gent^R gene; oriC, M. agalactiae origin of replication. B. Southern blot analysis of xer1-complemented PLMU. ClaI digest of DNA from the complementation clone PLMU-CP (lane 4) is shown in comparison with wt strain PG2 (lane 1), PLMU (lane 2) and complementation plasmid p21-7Gxer (lane 3) after hybridization with a xer1-specific DIG-labelled probe. G represents the 13 kb ClaI fragment of M. agalactiae type strain PG2 whereas ΔXERA and ΔXERB represent the two ClaI fragments from PLMU (as described in Fig. 3); P_CP depicts the 9.5 kb linearized complementation plasmid p21-7Gxer. DNA size standards are indicated in the left margin.