Fig. 2.

Characterization of di-cistronic reporter. A. (Top) fLuc activity in extracts of E. coli CA274 cells transformed with the wild-type di-cistronic reporter (CL) or the empty vector (vector), induced with increasing concentrations of arabinose. fLuc activity is reported as relative luminescence units (RLU)/OD₆₀₀. (Bottom) Immunoblots of cell extracts with anti-CAT or anti-fLuc antibody (Ab). B. Immunoblots with anti-CAT or anti-fLuc Ab of E. coli CA274 cell extracts containing empty vector, CL, C_am1L or CL_am1.