Fig. 5.
De novo and re-initiation efficiency of mutant initiator tRNAs. A. Relative CAT activity from E. coli CA274 transformed with the Cam1L reporter, expressing a mutant CAT gene initiating with a UAG codon and the U35A36 mutant initiator tRNA (grey bar) or the U35A36 mutant tRNA containing additional mutations as noted (white bars). Activity from the U35A36 mutant initiator tRNA was set at 100% and CAT activity defined as in legend to Fig. 4A. B. Relative fLuc activity from E. coli CA274 transformed with the CLam1 reporter, expressing a mutant fLuc gene initiating with a UAG codon, and the U35A36 mutant initiator tRNA (grey bar) or the U35A36 mutant initiator tRNA containing additional mutations as noted (white bars). Activity from the U35A36 mutant initiator tRNA was set at 100% and fLuc activity is as defined in legend to Fig. 4A.