Fig. 8.
Overexpression of IF3 reduces re-initiation efficiency. A and B. E. coli CA274 cells were co-transformed with CL and expression plasmids that either were empty (−), or contained genes encoding IF2, IF3, MetRS or MTF. Cell extracts were analysed using immunoblots with anti-β-lactamase or anti-CAT Ab (A), or anti-CAT and anti-fLuc Ab (B). C. E. coli CA274 cells were co-transformed with CL or CrbsL, and a compatible expression plasmid that either was empty or contained the IF3 gene. fLuc activity is presented as percentage change in fLuc activity in cells overexpressing IF3, relative to cells not overexpressing IF3. fLuc activity is as defined in legend to Fig. 4A.