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. Author manuscript; available in PMC: 2008 Mar 19.
Published in final edited form as: Antioxid Redox Signal. 2008 Feb;10(2):269–275. doi: 10.1089/ars.2007.1910

FIG. 1. Histology and Masson's Trichrome staining of the lung after exposure to hyperoxia or instillation of intratracheal LPS.

FIG. 1

Hematoxylin and eosin (H&E) staining or Masson's trichrome staining were performed on paraffin-fixed lung sections. (A) Mice were exposed to hyperoxia (FiO2 1.0) for 72 h. Light microscopy showed acute lung inflammation with numerous inflammatory cells, mainly polymorphonuclear leukocytes, and erythocytes within the alveolar spaces and interstitium. (B) Masson's Trichrome staining showed patchy collagen deposition (blue staining) within the alveolar spaces in some areas. (C) H&E and (D) Masson's Trichome after LPS (1 mg/kg) was instilled via intratracheal instillation, and lungs were fixed 72 h later. Note the intra-alveolar and interstitial inflammatory cell accumulation, as well as the scarcity of alveolar collagen deposition. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article at www.liebertonline.com/ars).