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. Author manuscript; available in PMC: 2008 Mar 19.
Published in final edited form as: Antioxid Redox Signal. 2008 Feb;10(2):269–275. doi: 10.1089/ars.2007.1910

FIG. 3. Fluorescence microscopy of lungs of mtGFP-tg mice after one of four inhalational injuries.

FIG. 3

(A) Mice were exposed continuously to hyperoxia (FiO2 1.0) for 72 h. Microscopy showed intense mitochondrial fluorescence in the smooth muscle of small blood vessels, along with scattered fluorescence in the inflammatory cells in the alveolar region. (B) LPS (1 mg/kg) was instilled via intratracheal route, and lungs were fixed after 72 h. Intense mitochondrial fluorescence was present in the inflammatory cells. (C) Asbestos (0.1 mg) was instilled via intratracheal route, and lungs were fixed 14 days after intratracheal instillation of asbestos. There was a similar pattern of fluorescence in vascular smooth muscle and sparsely within the alveolar region. (D) Bleomycin (2 mg/kg) was instilled via intratracheal route, and lungs were fixed after 14 days. Small muscularized pulmonary vessels showed substantial increase in green fluorescence, primarily in the smooth muscle, inflammatory cells, and within the expanded interstitium. (E) Mitochondrial distribution in the lung of unexposed control mtGFP-tg mouse. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article at www.liebertonline.com/ars).