Figure 5.
Phosphorylation of STAT3 induced by vIL-10 isoforms. Media from 293FT cells transfected with vIL-10 expressing plasmids were used to induce STAT3 phosphorylation in THP-1 cells. THP-1 cells were treated with the conditioned media for 1.5 hours, and then subjected to Western blot analysis with an anti-phosphorylated STAT3 (Tyr705) or an anti-STAT3 antibody. Expressions of vIL-10 isoforms were included to indicated the input of each protein.