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Interaction of vIL-10 isoforms and hIL-10. Each vIL-10 isoforms was co-expressed with GST or hIL-10-GST in 293FT cells. Cell lysates were subjected to a GST pull-down assay. Samples were then analyzed by Western blot with an anti-myc antibody (upper panels). The whole cell lysates (WCL) were also analyzed to show equal amounts of vIL-10 isoform in each sample (lower panels).
