Figure 1.

Cytokine production of T1/ST2⁺ Th cells in vitro. (A) Spleen cells from DO11.10 TCR-transgenic mice were primed in vitro in the presence or absence of IL-4 and anti-IL-12. One week later, the T cells were restimulated in vitro with peptide and antigen-presenting cells. Again, one culture received IL-4 and anti-IL-12 for further Th2 polarization, whereas the other culture did not receive additional cytokines. Ten days later, Th cells from both cultures were mixed, stained with anti-T1/ST2 mAb using magnetofluorescent liposomes, and sorted by MACS. Percentages of T1/ST2⁺ cells are indicated. (B) Unsorted cells and the T1/ST2-enriched or -depleted fractions were stimulated with PMA/ionomycin. Twenty-four hours later the cytokine concentrations in the culture supernatants were determined by ELISA. Values for IL-10 in the unsorted and depleted cell fractions were below the detection limit (<DL) of 0.5 ng/ml.