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Antigen-experienced but not naive Th cells express T1/ST2. Spleen cells from BALB/c mice were stained with mAbs against T1/ST2, CD4, and CD62L. Gates were set on viable cells according to forward and sideward scatter and exclusion of propidium iodide-binding particles. By gating on CD62Lhi or CD62Llo CD4+ cells, histograms for 3E10 staining were obtained.
