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Cytokine production of T1/ST2+ cells ex vivo. T1/ST2+ lymphocytes from spleens and lymph nodes of BALB/c mice were enriched by MACS. Enriched and depleted cell fractions were stimulated for 5 hr with PMA/ionomycin, fixed, and stained for CD4 and intracellular cytokines. For analysis, gates were set on CD4+ lymphocytes. Equal numbers of CD4+ cells are depicted in each dot plot.
