Figure 5.

T1/ST2⁺ cells in IL-4^−/− mice. (A) Spleen cells from wt and IL-4^−/− BALB/c mice were stained with mAbs against T1/ST2, CD4, and CD62L as described for Fig. 2. The control panel shows the fluorescence signal of anti-DIG-Cy5 alone. To verify its specificity, the staining of 3E10 was blocked by preincubation of the cells with a 100-fold excess of unconjugated 3E10, which reduced the subsequent 3E10 staining on CD4⁺ cells to control levels in both wt and IL-4^−/− mice. The dot plots represent the results obtained with IL-4^−/− mice. (For wt BALB/c mice see Fig. 2.) (B) Frequency of T1/ST2⁺ cells among CD4⁺ cells from wt and IL-4^−/− mice, as determined in A.