Figure 6.

Cytokine production of T1/ST2⁺ cells from IL-4^−/− mice ex vivo. Lymphocytes from spleens of IL-4^−/− mice were stained with anti-T1/ST2 mAb and sorted by MACS. Enriched and depleted cell fractions were stimulated for 5 hr with PMA/ionomycin, fixed, and stained for CD4 and intracellular cytokines. For analysis, gates were set on CD4⁺ lymphocytes. Equal numbers of CD4⁺ cells are depicted in each dot plot.