Skip to main content
. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2007 Nov 17;227(2):257–264. doi: 10.1016/j.taap.2007.10.025

Fig. 4.

Fig. 4

Effect of overexpression ans depletion of GSTA4 on genotoxic effects induced by HNE in K562 cells. Stable mGSTA4-4 transfected K562 cells were treated with 5 μM (A) and 10 μM (B) of HNE for 1, 3 and 6 h followed by single cell gel-electrophoresis to assess the DNA damage. (C) The GSTA4-4 siRNA transfected cells and control siRNA transfected cells were treated with 5 and 10 μM of HNE for 3 h and comet assay was performed to assess the DNA damage. The bars show tail intensities (mean ± S.D.) from three independent experiments, each mean was calculated from three to four parallel slides, 50 comets were evaluated per slide. *P < 0.01 vs 5 μM HNE for 6 h; **P < 0.05 vs 10 μM HNE for 3 h; #P< 0.01 vs 10 μM HNE for 6 h; # #P < 0.01 vs respective Csi groups. The inset in figures represents Western blots for GSTA4-4 proteins in wild type (WT), and mGSTA4-4 (A and B) and control- (Csi) GSTA4-4 -siRNA (GSTA4si) (C) transfected K562 cells.