Table 1.
Properties of the p53 tetramerization domain mutants
| Mutant | WT AA | Mutant AA | Polony μ (h−1) | Red/White | %WT activity | p53 Western blot |
|---|---|---|---|---|---|---|
| Leu330Ala | Hydrophobic uncharged | – | 3.7 ± 0.6 | 5.6 ± 4.2 | – | |
| Leu330Arg | Hydrophilic basic | – | 4.3 ± 0.6 | – | – | |
| Leu330Asn | Hydrophilic uncharged | – | 4.3 ± 0.6 | 7.2 ± 14.4 | – | |
| Leu330Asp | Hydrophilic acidic | – | 5.0 ± 0 | 47.6 ± 8.0 | 121.7% ± 8.3% | |
| Leu330Cys | Uncharged hydrophobic | 0.174 | 1.7 ± 0.6 | – | – | |
| Leu330Gln | Hydrophilic uncharged | – | 3.0 ± 0 | – | – | |
| Leu330Glu | Hydrophilic acidic | – | 5.0 ± 0 | 54.4 ± 22.3 | – | |
| Leu330Gly | Uncharged | – | 5.0 ± 0 | 5.5 ± 5.4 | – | |
| Leu330His | Hydrophilic basic | – | 4.3 ± 0.6 | 7.6 ± 10.0 | – | |
| Leu330Ile | Hydrophobic uncharged | 0.196 | 1.3 ± 0.6 | 250.5 ± 7.4 | – | |
| Leu330Lys | Hydrophilic basic | – | 5.0 ± 0 | 29.9 ± 16.2 | – | |
| Leu330Met | Hydrophobic uncharged | 0.205 | 2.0 ± 1.0 | 72.8 ± 4.5 | – | |
| Leu330Phe | Hydrophobic uncharged | 0.192 | 1.0 ± 0 | – | 88.9% ± 11.3% | |
| Leu330Pro | Uncharged | – | 4.0 ± 0 | – | – | |
| Leu330Ser | Hydrophilic uncharged | 0.186 | 4.7 ± 0.6 | – | – | |
| Leu330Thr | Hydrophilic uncharged | 0.193 | 4.3 ± 0.6 | – | – | |
| Leu330Trp | Hydrophobic uncharged | – | 4.0 ± 0 | – | 98.4% ± 7.3% | |
| Leu330Tyr | Hydrophobic ionizable | 0.198 | 1.3 ± 0.6 | 150.6 ± 27.9 | 100.0% | |
| Leu330Val | Hydrophobic uncharged | Hydrophobic uncharged | 0.195 | 1.3 ± 0.6 | 164.9 ± 22.6 | – |
| Gln331Ala | Hydrophobic uncharged | 0.205 | 1.0 ± 0 | 77.6 ± 8.0 | – | |
| Gln331Arg | Hydrophilic basic | 0.202 | 1.7 ± 0.6 | – | – | |
| Gln331Asn | Hydrophilic uncharged | 0.203 | 2.0 ± 0 | – | 80.5% ± 17.1% | |
| Gln331Asp | Hydrophilic acidic | 0.211 | 1.3 ± 0.6 | – | – | |
| Gln331Cys | Uncharged hydrophobic | – | 3.0 ± 0 | – | – | |
| Gln331Glu | Hydrophilic uncharged | 0.203 | 2.0 ± 0 | – | – | |
| Gln331Gly | Hydrophilic acidic | 0.203 | 1.7 ± 0.6 | – | – | |
| Gln331His | Uncharged | 0.217 | 1.0 ± 0 | 153.0 | – | |
| Gln331Ile | Hydrophilic basic | 0.204 | 2.0 ± 0 | – | – | |
| Gln331Leu | Hydrophobic uncharged | 0.209 | 2.0 ± 0 | – | – | |
| Gln331Lys | Hydrophilic basic | 0.199 | 1.0 ± 0 | – | – | |
| Gln331Met | Hydrophobic uncharged | 0.203 | 1.3 ± 0.6 | – | – | |
| Gln331Phe | Hydrophobic uncharged | 0.186 | 2.0 ± 0 | 46.3 | – | |
| Gln331Pro | Uncharged | 0.197 | 3.3 ± 0.6 | 37.9 ± 13.2 | – | |
| Gln331Ser | Hydrophilic uncharged | 0.184 | 1.0 ± 0 | – | – | |
| Gln331Thr | Hydrophilic uncharged | 0.194 | 2.0 ± 0 | 56.2 ± 6.8 | – | |
| Gln331Trp | Hydrophobic uncharged | 0.192 | 4.0 ± 0 | 107.7 ± 14.7 | – | |
| Gln331Tyr | Hydrophobic ionizable | – | 2.7 ± 0.6 | – | – | |
| Gln331Val | Hydrophilic uncharged | Hydrophobic uncharged | – | 2.3 ± 0.6 | – | – |
| Ile332 Ala | Hydrophobic uncharged | – | 4.3 ± 0.6 | 14.7 | – | |
| Ile332Arg | Hydrophilic basic | – | 5.0 ± 0 | – | – | |
| Ile332Asn | Hydrophilic uncharged | 0.205 | 5.0 ± 0 | 33.1 | – | |
| Ile332Asp | Hydrophilic acidic | – | 5.0 ± 0 | – | – | |
| Ile332Cys | Uncharged hydrophobic | 0.202 | 1.0 ± 0 | – | 86.1% ± 6.9% | |
| Ile332Gln | Hydrophilic uncharged | – | 5.0 ± 0 | – | – | |
| Ile332Glu | Hydrophilic acidic | – | 5.0 ± 0 | 26.9 ± 6.9 | 122.2% ± 25.5% | |
| Ile332Gly | Uncharged | – | 4.0 ± 0 | – | – | |
| Ile332His | Hydrophilic basic | – | 5.0 ± 0 | – | 110.9% ± 12.0% | |
| Ile332Leu | Hydrophobic uncharged | 0.198 | 1.0 ± 0 | 84.2 | – | |
| Ile332Lys | Hydrophilic basic | – | 5.0 ± 0 | – | – | |
| Ile332Met | Hydrophobic uncharged | 0.190 | 2.0 ± 0 | 179.2 ± 35.8 | – | |
| Ile332 Phe | Hydrophobic uncharged | – | 5.0 ± 0 | – | – | |
| Ile332Pro | Uncharged | – | 5.0 ± 0 | – | – | |
| Ile332Ser | Hydrophilic uncharged | – | 4.7 ± 0.6 | 35.4 | – | |
| Ile332Thr | Hydrophilic uncharged | 0.192 | 2.3 ± 0.6 | – | 101.0% ± 6.6% | |
| Ile332Trp | Hydrophobic uncharged | – | 4.7 ± 0.6 | – | – | |
| Ile332Tyr | Hydrophobic ionizable | – | 4.7 ± 0.6 | – | – | |
| Ile332Val | Hydrophobic uncharged | Hydrophobic uncharged | 0.198 | 2.7 ± 0.6 | 56.0 | – |