Name	Sequence	Purpose
272	AAACACAAATACACACACTAATCTAATGGAGGAGCCGCAGTCAGATCCTAGCGTC	Common primer for p53, 5′ end
271	TAAATTACTATACTTCTATAGACACGCAAACACAAATACACACACTAATCTAATG	Mutant p53 ampli-fication and addition of restriction sites
278	CAGAGCTTGTGGGGGTTCACCCACTTGTAGGTGCCCTCATACTGGTCAGTC
276	CGTGGGCGTGAGCGCTTCGAGATGTTCCGA	Primers for p53, 3′ end
277	CACTTGTAGGTGCCCTCATACTGGTCAGTCTGAGTCAGGCCCTTCTGTCTTGAAC
221	CCAGTATGAGGGCACCTACAAGTGGGTGAACCCC	DNA sequence tag synthesis
222	CAAGCTTTCAGAGCTTGTGGGGGTTCACCCACTTGTAGGTGCCCTCATACTGG
223	CACAAGCTCTGAAAGCTTGAGTAACGGGTCTTGTTCGC
224	GCACTGCACTGGTGACCGGCGAACAAGACCCGTTAC
225	GGTCACCAGTGCAGTGCTGCGTCTTCACGGACTTC
226	GAGTACGAGGTGATCTCCGGAAGTCCGTGAAGACGC
227	GGAGATCACCTCGTACTCTGATTGCTGTGCAGCTCAC
228	AGAGACCTAACAGTAGGGAAACTGTGAGCTGCACAGCAATC
229	GTTTCCCTACTGTTAGGTCTCTCCATGCTACACTCGTCGACAGCTGA
230	TCAGCTGTCGACGAGTGTAGCATGG
238	CACAAGCTCTGAAAGCTTGTGTAACGGGTCTTGTTCGC
239	GCACTGCACTGGTGACCTGCGAACAAGACCCGTTAC
240	GGTCACCAGTGCAGTGCCGCGTCTTCACGGACTTC
241	GAGTACGAGGTGATCTCCTGAAGTCCGTGAAGACGC
242	GGAGATCACCTCGTACTCAGATTGCTGTGCAGCTCAC
243	AGAGACCTAACAGTAGGGAAACAGTGAGCTGCACAGCAATC
175	CAACCGCCTCTTCTACCTGG	Colony PCR with 230
249B	GGGGACTAGTATGGAGGAGCCGCAGTCAGATC	p53 isolation
249C	GGGGAAGCTTTCAGTCTGAGTCAGGCCCTT
327	CTACATGTGTAACAGTTCCTGCATGGGC	p53 mRNA expression analysis
328	[5Acrd]TTCTTTGGCTGGGGAGAGGAGC
329	AGGGAGCACTAAGCGAGCAC
333	AAACAATTATCGCTGGAGCTGGTGGG	ADE2 mRNA expression analysis
334	[5Acrd]AAGCGCCAAGCAGTCTGACAGC
335	CACCACTTCCTGTCATCGGTGTG
325	CTCACTCCAGCCACCTGAAGTCCAA	Sequence tag polony amplification
326	[5Acrd]GAGTGTAGCATGGAGAGACCTAACAGTAG
324	GGCCTGACTCAGACTGAAAGCTTG	Sequence tag single base extension
252	GTAACGGGTCTTGTTCGC
253	GGTCACCAGTGCAGTGC
254	GCGTCTTCACGGACTTC
255	GGAGATCACCTCGTACTC
256	GATTGCTGTGCAGCTCAC